ypd or ypgal medium Search Results


rich medium ypd  (US Biological Life Sciences)
90
US Biological Life Sciences rich medium ypd
Rich Medium Ypd, supplied by US Biological Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1% bacto-yeast extract  (Bacto Laboratories)
90
Bacto Laboratories 1% bacto-yeast extract
1% Bacto Yeast Extract, supplied by Bacto Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1% (w/v) bactotm peptone  (Bacto Laboratories)
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Bacto Laboratories 1% (w/v) bactotm peptone
1% (W/V) Bactotm Peptone, supplied by Bacto Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ypgal (1% yeast extract, 2% peptone and 2% galactose)  (Difco)
90
Difco ypgal (1% yeast extract, 2% peptone and 2% galactose)
Ypgal (1% Yeast Extract, 2% Peptone And 2% Galactose), supplied by Difco, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ypgal  (Bacto Laboratories)
90
Bacto Laboratories ypgal
Ypgal, supplied by Bacto Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ypgal (1% yeast/2% peptone/2% galactose)  (Difco)
90
Difco ypgal (1% yeast/2% peptone/2% galactose)
Expression of Arabidopsis vacuolar pyrophosphatase AVP1 in ena1 mutants. (A) Vector pYES2 (Invitrogen) was introduced into wild-type, ena1, ena1 nhx1, and ena1 gef1 mutants. Plasmid pYes2-AVP1-D (13) was introduced into ena1, ena1 nhx1, and ena1 gef1 mutants. Five-fold serial dilutions (starting at 105 cells) of each strain were plated on <t>YPGAL</t> (1% yeast extract/2% <t>peptone/2%</t> <t>galactose)</t> with or without 0.5 M NaCl and incubated at 30°C for 2 days. (B and C) Intracellular concentrations of Na+ and K+. Exponentially growing cells (wild-type and ena1 transformed with pYES2 vector and ena1,ena1 nhx1, and ena1 gef1 mutants carrying pYes2-AVP1-D) were exposed to 0.7 M NaCl for 6 h. Total cell extracts were prepared (see Materials and Methods), and Na+ and K+ concentrations were determined. Values are the mean of two determinations, and bars represent the standard deviations. There is a consistent reduction in total cell Na+ in the ena1 AVP-D strain. The reason for this reduction is unknown.
Ypgal (1% Yeast/2% Peptone/2% Galactose), supplied by Difco, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bacto-peptone  (Bacto Laboratories)
90
Bacto Laboratories bacto-peptone
Expression of Arabidopsis vacuolar pyrophosphatase AVP1 in ena1 mutants. (A) Vector pYES2 (Invitrogen) was introduced into wild-type, ena1, ena1 nhx1, and ena1 gef1 mutants. Plasmid pYes2-AVP1-D (13) was introduced into ena1, ena1 nhx1, and ena1 gef1 mutants. Five-fold serial dilutions (starting at 105 cells) of each strain were plated on <t>YPGAL</t> (1% yeast extract/2% <t>peptone/2%</t> <t>galactose)</t> with or without 0.5 M NaCl and incubated at 30°C for 2 days. (B and C) Intracellular concentrations of Na+ and K+. Exponentially growing cells (wild-type and ena1 transformed with pYES2 vector and ena1,ena1 nhx1, and ena1 gef1 mutants carrying pYes2-AVP1-D) were exposed to 0.7 M NaCl for 6 h. Total cell extracts were prepared (see Materials and Methods), and Na+ and K+ concentrations were determined. Values are the mean of two determinations, and bars represent the standard deviations. There is a consistent reduction in total cell Na+ in the ena1 AVP-D strain. The reason for this reduction is unknown.
Bacto Peptone, supplied by Bacto Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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yeast extract bacto peptone  (Bacto Laboratories)
90
Bacto Laboratories yeast extract bacto peptone
Expression of Arabidopsis vacuolar pyrophosphatase AVP1 in ena1 mutants. (A) Vector pYES2 (Invitrogen) was introduced into wild-type, ena1, ena1 nhx1, and ena1 gef1 mutants. Plasmid pYes2-AVP1-D (13) was introduced into ena1, ena1 nhx1, and ena1 gef1 mutants. Five-fold serial dilutions (starting at 105 cells) of each strain were plated on <t>YPGAL</t> (1% yeast extract/2% <t>peptone/2%</t> <t>galactose)</t> with or without 0.5 M NaCl and incubated at 30°C for 2 days. (B and C) Intracellular concentrations of Na+ and K+. Exponentially growing cells (wild-type and ena1 transformed with pYES2 vector and ena1,ena1 nhx1, and ena1 gef1 mutants carrying pYes2-AVP1-D) were exposed to 0.7 M NaCl for 6 h. Total cell extracts were prepared (see Materials and Methods), and Na+ and K+ concentrations were determined. Values are the mean of two determinations, and bars represent the standard deviations. There is a consistent reduction in total cell Na+ in the ena1 AVP-D strain. The reason for this reduction is unknown.
Yeast Extract Bacto Peptone, supplied by Bacto Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ypgal media  (Difco)
90
Difco ypgal media
Expression of Arabidopsis vacuolar pyrophosphatase AVP1 in ena1 mutants. (A) Vector pYES2 (Invitrogen) was introduced into wild-type, ena1, ena1 nhx1, and ena1 gef1 mutants. Plasmid pYes2-AVP1-D (13) was introduced into ena1, ena1 nhx1, and ena1 gef1 mutants. Five-fold serial dilutions (starting at 105 cells) of each strain were plated on <t>YPGAL</t> (1% yeast extract/2% <t>peptone/2%</t> <t>galactose)</t> with or without 0.5 M NaCl and incubated at 30°C for 2 days. (B and C) Intracellular concentrations of Na+ and K+. Exponentially growing cells (wild-type and ena1 transformed with pYES2 vector and ena1,ena1 nhx1, and ena1 gef1 mutants carrying pYes2-AVP1-D) were exposed to 0.7 M NaCl for 6 h. Total cell extracts were prepared (see Materials and Methods), and Na+ and K+ concentrations were determined. Values are the mean of two determinations, and bars represent the standard deviations. There is a consistent reduction in total cell Na+ in the ena1 AVP-D strain. The reason for this reduction is unknown.
Ypgal Media, supplied by Difco, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ypgal medium  (Bacto Laboratories)
90
Bacto Laboratories ypgal medium
Expression of Arabidopsis vacuolar pyrophosphatase AVP1 in ena1 mutants. (A) Vector pYES2 (Invitrogen) was introduced into wild-type, ena1, ena1 nhx1, and ena1 gef1 mutants. Plasmid pYes2-AVP1-D (13) was introduced into ena1, ena1 nhx1, and ena1 gef1 mutants. Five-fold serial dilutions (starting at 105 cells) of each strain were plated on <t>YPGAL</t> (1% yeast extract/2% <t>peptone/2%</t> <t>galactose)</t> with or without 0.5 M NaCl and incubated at 30°C for 2 days. (B and C) Intracellular concentrations of Na+ and K+. Exponentially growing cells (wild-type and ena1 transformed with pYES2 vector and ena1,ena1 nhx1, and ena1 gef1 mutants carrying pYes2-AVP1-D) were exposed to 0.7 M NaCl for 6 h. Total cell extracts were prepared (see Materials and Methods), and Na+ and K+ concentrations were determined. Values are the mean of two determinations, and bars represent the standard deviations. There is a consistent reduction in total cell Na+ in the ena1 AVP-D strain. The reason for this reduction is unknown.
Ypgal Medium, supplied by Bacto Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rich medium yeast extract-peptone-dextrose [ypd] or ypgal  (US Biological Life Sciences)
90
US Biological Life Sciences rich medium yeast extract-peptone-dextrose [ypd] or ypgal
Expression of Arabidopsis vacuolar pyrophosphatase AVP1 in ena1 mutants. (A) Vector pYES2 (Invitrogen) was introduced into wild-type, ena1, ena1 nhx1, and ena1 gef1 mutants. Plasmid pYes2-AVP1-D (13) was introduced into ena1, ena1 nhx1, and ena1 gef1 mutants. Five-fold serial dilutions (starting at 105 cells) of each strain were plated on <t>YPGAL</t> (1% yeast extract/2% <t>peptone/2%</t> <t>galactose)</t> with or without 0.5 M NaCl and incubated at 30°C for 2 days. (B and C) Intracellular concentrations of Na+ and K+. Exponentially growing cells (wild-type and ena1 transformed with pYES2 vector and ena1,ena1 nhx1, and ena1 gef1 mutants carrying pYes2-AVP1-D) were exposed to 0.7 M NaCl for 6 h. Total cell extracts were prepared (see Materials and Methods), and Na+ and K+ concentrations were determined. Values are the mean of two determinations, and bars represent the standard deviations. There is a consistent reduction in total cell Na+ in the ena1 AVP-D strain. The reason for this reduction is unknown.
Rich Medium Yeast Extract Peptone Dextrose [Ypd] Or Ypgal, supplied by US Biological Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rich medium yeast extract-peptone-dextrose [ypd] or ypgal - by Bioz Stars, 2026-02
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ypgal  (Difco)
90
Difco ypgal
Expression of Arabidopsis vacuolar pyrophosphatase AVP1 in ena1 mutants. (A) Vector pYES2 (Invitrogen) was introduced into wild-type, ena1, ena1 nhx1, and ena1 gef1 mutants. Plasmid pYes2-AVP1-D (13) was introduced into ena1, ena1 nhx1, and ena1 gef1 mutants. Five-fold serial dilutions (starting at 105 cells) of each strain were plated on <t>YPGAL</t> (1% yeast extract/2% <t>peptone/2%</t> <t>galactose)</t> with or without 0.5 M NaCl and incubated at 30°C for 2 days. (B and C) Intracellular concentrations of Na+ and K+. Exponentially growing cells (wild-type and ena1 transformed with pYES2 vector and ena1,ena1 nhx1, and ena1 gef1 mutants carrying pYes2-AVP1-D) were exposed to 0.7 M NaCl for 6 h. Total cell extracts were prepared (see Materials and Methods), and Na+ and K+ concentrations were determined. Values are the mean of two determinations, and bars represent the standard deviations. There is a consistent reduction in total cell Na+ in the ena1 AVP-D strain. The reason for this reduction is unknown.
Ypgal, supplied by Difco, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ypgal - by Bioz Stars, 2026-02
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Image Search Results


Expression of Arabidopsis vacuolar pyrophosphatase AVP1 in ena1 mutants. (A) Vector pYES2 (Invitrogen) was introduced into wild-type, ena1, ena1 nhx1, and ena1 gef1 mutants. Plasmid pYes2-AVP1-D (13) was introduced into ena1, ena1 nhx1, and ena1 gef1 mutants. Five-fold serial dilutions (starting at 105 cells) of each strain were plated on YPGAL (1% yeast extract/2% peptone/2% galactose) with or without 0.5 M NaCl and incubated at 30°C for 2 days. (B and C) Intracellular concentrations of Na+ and K+. Exponentially growing cells (wild-type and ena1 transformed with pYES2 vector and ena1,ena1 nhx1, and ena1 gef1 mutants carrying pYes2-AVP1-D) were exposed to 0.7 M NaCl for 6 h. Total cell extracts were prepared (see Materials and Methods), and Na+ and K+ concentrations were determined. Values are the mean of two determinations, and bars represent the standard deviations. There is a consistent reduction in total cell Na+ in the ena1 AVP-D strain. The reason for this reduction is unknown.

Journal:

Article Title: The Arabidopsis thaliana proton transporters, AtNhx1 and Avp1, can function in cation detoxification in yeast

doi:

Figure Lengend Snippet: Expression of Arabidopsis vacuolar pyrophosphatase AVP1 in ena1 mutants. (A) Vector pYES2 (Invitrogen) was introduced into wild-type, ena1, ena1 nhx1, and ena1 gef1 mutants. Plasmid pYes2-AVP1-D (13) was introduced into ena1, ena1 nhx1, and ena1 gef1 mutants. Five-fold serial dilutions (starting at 105 cells) of each strain were plated on YPGAL (1% yeast extract/2% peptone/2% galactose) with or without 0.5 M NaCl and incubated at 30°C for 2 days. (B and C) Intracellular concentrations of Na+ and K+. Exponentially growing cells (wild-type and ena1 transformed with pYES2 vector and ena1,ena1 nhx1, and ena1 gef1 mutants carrying pYes2-AVP1-D) were exposed to 0.7 M NaCl for 6 h. Total cell extracts were prepared (see Materials and Methods), and Na+ and K+ concentrations were determined. Values are the mean of two determinations, and bars represent the standard deviations. There is a consistent reduction in total cell Na+ in the ena1 AVP-D strain. The reason for this reduction is unknown.

Article Snippet: Cells were grown in YPD (1% yeast/2% peptone/2% dextrose; Difco), YPGAL (1% yeast/2% peptone/2% galactose; Difco), SD (Difco; Synthetic medium with 2% Dextrose), or APG (APG is a synthetic minimal medium containing 10 mM arginine, 8 mM phosphoric acid, 2% glucose, 2 mM MgSO 4 , 1 mM KCl, 0.2 mM CaCl 2 , and trace minerals and vitamins) ( 12 ).

Techniques: Expressing, Plasmid Preparation, Incubation, Transformation Assay